SU-DHL-8 is a B lymphocyte cell line, isolated in 1978 from the lymph node of a 59-year-old Caucasian male with large cell lymphoma.

Cell Name: Human diffuse large B-cell lymphoma cell line

Cell Synonyms: SU-DHL-8; SUDHL8; SUDHL-8; SuDHL 8; Stanford University-Diffuse Histiocytic Lymphoma-8; DHL-8; DHL8

Catalogue No.: TCH-C516
Species: Human
Tissue Origin: Lymph Node
Disease Characteristic: Large Cell Lymphoma
Morphology: Epithelial-like
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G516

Passage Ratio: 1:2-1:10, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection