This cell line is derived from the ascites of a 43-year-old male and consists of B lymphocytes. It is Epstein-Barr virus (EBV)-negative and carries the t(14;18) translocation.

Cell Name: Human diffuse large B-cell lymphoma cell line

Cell Synonyms: SU-DHL-6; SUDHL6; SuDHL6; SU-DHL6; SUDHL-6; SuDHL-6; Sudhl-6; SUD6; Stanford University-Diffuse Histiocytic Lymphoma-6; DHL-6; DHL6

Catalogue No.: TCH-C506
Species: Human
Tissue origin: Ascites fluid
Disease Characteristics: Lymphoma cells
Morphology: Lymphoblast-like
Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G506

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection