SNU-16 is a cell line exhibiting epithelial morphology, isolated in 1987 from the ascites of a 33-year-old Asian female gastric cancer patient prior to chemotherapy. These cells are used in cancer research. The cell line was established from cells collected before chemotherapy. SNU-16 cells are positive for VIP receptors but lack gastrin receptors.

The cell line was established from cells collected before chemotherapy. No evidence of amplification or rearrangement was found in the N-myc, L-myc, myb, or EGF receptor genes. The c-myc proto-oncogene was amplified, but the expressed c-erb-B-2 RNA was comparable to other cell lines. The following genes were not expressed: N-myc, L-myc, c-cis, IGF-2, or gastrin-releasing peptide. The cells express the surface glycoprotein carcinoembryonic antigen (CEA) and TAG-72. The cells are positive for L-dopa decarboxylase (DDC).

Cell Name: Human gastric carcinoma cell line

Cell Synonyms: SNU-16; SNU16; NCI-SNU-16

Catalogue No.: TCH-C505
Species: Human
Tissue Origin: Stomach
Disease Characteristics: Gastric Cancer
Morphology: Epithelial-like
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G505

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection