The human gastric carcinoma cell line NCI-N87   expresses the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72, but does not express L-dopa decarboxylase (DDC). These cells exhibit very low activity of vasoactive intestinal peptide (VIP) receptors and lack gastrin receptors. They do express muscarinic cholinergic receptors. There is no evidence of rearrangement in the genes for N-myc, L-myc, myb, or the EGF receptor. The expression levels of c-myc and c-erb-B2 RNA in this cell line are comparable to those in other cell lines. The following genes are not expressed: N-myc, L-myc, c-cis, IGF-2, or gastrin-releasing peptide. It has been reported that the plating efficiency of NCI-N87 cells is 4.3%.

Cell Name: Human gastric carcinoma cell line

Cell Synonyms: NCI-N87; NCI N87;N87;N-87;NCI-H87;H87;H-87;NCIN87

Catalogue No.: TCH-C504
Species: Human
Tissue Origin: Stomach
Disease Characteristic: Gastric Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Matching Medium Catalog Number: TCH-G504

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 48-80 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection