In 1987, J. Park and colleagues established the SNU-5 cell line from the ascites of a patient with poorly differentiated gastric cancer. The patient had previously received chemotherapy including 5-fluorouracil, doxorubicin, and mitomycin.

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72.

The cells are positive for L-dopa decarboxylase (DDC).

SNU-5 cells are positive for VIP receptors but lack gastrin receptors. They express muscarinic cholinergic factor receptors. There is no evidence of amplification or rearrangement of the N-myc, L-myc, myb, or EGF receptor genes. The expression levels of c-myc and c-erb-B-2 RNA in these cells are comparable to those in other cell lines. The following genes are not expressed: N-myc, L-myc, c-cis, IGF-2, or gastrin-releasing peptide.

Cell Name: Human gastric carcinoma cell line

Cell Synonyms: SNU-5
Catalogue No.: TCH-C503
Species: Human
Tissue Origin: Stomach
Disease Characteristics: Gastric Cancer
Morphology: Epithelial-like
Growth Properties: Suspension
Culture Medium: IMDM + 20% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G503

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection