LS 180 is a cell line exhibiting epithelial morphology, isolated from the colon of a 58-year-old Caucasian female patient with colorectal cancer.

Cell Name: Human colorectal adenocarcinoma cell line

Cell Abbreviation: LS_180; LS180; LS-180; LS 180; Laboratory of Surgery 180

Product Number: TCH-C501
Species: Human
Tissue Origin: Large intestine; Colon
Disease Characteristics: Adenocarcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM+10%FBS+1%NEAA+1%P/S

Matching Medium Catalog Number: TCH-G501
Passage Ratio: 1:2-1:5, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection