SNU-449 was established in 1990 by J.-G. Park and colleagues from a Korean patient undergoing cytotoxic therapy. The cell line was derived by Park et al. in 1990 from a primary hepatocellular carcinoma specimen obtained prior to cytotoxic treatment in a Korean patient.

Cell Name: Human hepatocellular carcinoma cell line

Cell Synonyms: SNU-449; SNU449; NCI-SNU-449

Catalogue No.: TCH-C500
Species: Human
Tissue Origin: Liver
Disease Characteristics: Hepatocellular Carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G500
Passage Ratio: 1:5-1:10, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection