This cell line was derived from the lymph node of a 48-year-old Caucasian female with non-small cell lung cancer. The patient was a smoker with a history of 60 packs per year. Each batch has been tested for mycoplasma contamination by our repository, with negative results.

Cell Name: Human non-small cell lung adenocarcinoma cell line

Cell Synonyms: NCI-H1568; H1568; H-1568; NCIH1568

Catalogue No.: TCH-C495

Species: Human
Tissue Origin: Lung
Disease Characteristics: Lung; derived from metastatic site: lymph node

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640+10%FBS+1%Glutamax+1% Sodium Pyruvate+ 1% P/S
Matching Medium Catalog Number: TCH-G495

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 42.5 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection