HCC2935 is an epithelial cell line that was isolated in 1999 from the pleural effusion of a White, 39-year-old, male patient with adenocarcinoma. This cell line was deposited by JD Minna, AF Gazdar.

Cell Name: Human non-small cell lung adenocarcinoma cell line

Cell Synonyms: HCC2935; HCC-2935; Hamon Cancer Center 2935

Catalogue No.: TCH-C489

Species: Human

Tissue Origin: Lung; Pleural Effusion

Disease Characteristics: Lung Adenocarcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G489
Passage Ratio: 1:4-1:6, with medium renewal every 2-3 days
Doubling Time: 70.4 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection