NCI-H2291 is a cell line exhibiting epithelial morphology that was isolated from the lungs of a male patient with small cell lung cancer: adenocarcinoma. This product has applications in cancer research.

Cell Name: Human non-small cell lung adenocarcinoma cell line

Cell Synonyms: NCI-H2291; H2291; H-2291; NCIH2291

Catalogue No.: TCH-C487

Species: Human
Tissue Origin: Lung
Disease Characteristics: Adenocarcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G487
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection