HCC1395 is an epithelial cell that was isolated from the mammary gland of a 43-year-old, White, female patient with ductal carcinoma TNM Stage I, grade 3. The cell was deposited by AF Gazdar and AK Virmani. It was initiated in 1994 and can be used in cancer research.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: HCC1395; HCC-1395; SCC-1395; Hamon Cancer Center 1395

Catalogue No.: TCH-C486
Species: Human
Tissue Origin: Thoracic; Breast
Disease Characteristics: Carcinoma; Ductal
Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G486
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 50-60 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection