HCC1599 is an epithelial cell that was isolated from the mammary gland of a 44-year-old, White, female patient with ductal carcinoma TNM stage IIIA, grade 3. The cell was deposited by AF Gazdar and AK Virmani and can be used in cancer research.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: HCC1599; HCC-1599; Hamon Cancer Center 1599

Catalogue No.: TCH-C485
Species: Human
Tissue Origin: Thoracic; Breast
Disease Characteristics: Ductal Carcinoma

Morphology: Lymphoblast-like

Growth Properties: Suspension

Culture Medium: RPMI-1640 + 20% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G485
Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection