SUM149PT was isolated from invasive ductal carcinoma of an ER-negative, PR-negative, and Her2-positive (non-activated) inflammatory breast cancer patient. This cell line is immortalized and expresses luminal cytokeratins 8, 18, and 19, consistent with its origin from luminal breast epithelial cells. It has been reported that SUM149PT can form tumors in nude mice.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: SUM149PT; SUM-149PT; SUM 149PT; SUM149-PT; SUM149; SUM-149; SUM 149; 149 PT; 149PT; BrCL12

Catalogue No.: TCH-C484
Species: Human
Tissue Origin: Breast
Disease Characteristics: Breast Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G484
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 84 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection