This cell line was established from a biopsy of a 70-year-old male with tongue squamous cell carcinoma. The squamous cell carcinoma consists of epithelioid cells that grow adherently in a monolayer.

Cell Name: Human tongue squamous cell carcinoma cell line

Cell Synonyms: SCC-25; SCC 25; SCC25; hSCC-25

Catalogue No.: TCH-C479

Species: Human

Tissue Origin: Tongue

Disease Characteristics: Squamous cell carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: D/F12+10%FBS+400ng/mL Hydrocortisone+ 1% P/S
Matching Medium Catalog Number: TCH-G479
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection