This cell line originates from Zhongshan Hospital and exhibits relatively slow growth.

Cell Name: Human low metastatic hepatocellular carcinoma cell line

Cell Synonyms: MHCC-97L; MHCC 97-L; MHCC97L

Catalogue No.: TCH-C476

Species: Human

Tissue Origin: Liver

Disease Characteristics: Liver Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G476
Passage Ratio: 1:2, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection