AU565 is an epithelial cell line isolated from the pleural effusion of a 43-year-old Caucasian female breast cancer patient. These cells can be used as a cellular growth model to study the differentiation of breast cancer cells.

Cell Name: Human breast adenocarcinoma cell line

Cell Synonyms: AU565; AU-565; AU 565

Product Number: TCH-C474
Species: Human
Tissue Origin: Pleura; Breast
Disease Characteristic: Adenocarcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G474

Passage Ratio: 1:4-1:6, with medium renewal every 3-5 days
Doubling Time: 46 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection