This cell line was isolated in 1977 by Cailleau R et al. from the pleural effusion of a 51-year-old African American woman with metastatic breast cancer. Although the donor tissue was heterozygous for the G6PD allele, this cell line consistently exhibits the G6PDA phenotype. There is a G→A mutation at codon 273 of the P53 gene, resulting in an Arg→His substitution. Each cell expresses 1×10⁶ EGF receptors.

Cell Name: Human breast adenocarcinoma cell line, cultured in DMEM-H (STR authenticated)
Cell Synonyms: MDA-MB-468; MDA-MB 468; MDA-MB468; MDAMB468; MDA-468; MDA468; MB468; MD Anderson-Metastatic Breast-468

Catalogue No.: TCH-C468
Species: Human
Tissue Origin: Breast
Disease Characteristic: Breast Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G468
Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 72-96 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection