Product Name：Luciferase Reporter SK-MEL-1 Cell Line

HyCyte cell line：HyCyte SK-MEL-1-Luc

Catalog No.：TCH-C461L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：SK-MEL-1

Species：Human

Morphology：Round

Growth properties：Suspension

Culture Medium：Dulbecco's Modified Eagle Medium ,High Glucose (DMEM) supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G461L）

Passage：1:2-1:4

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).