The parental cell line MM.1 of this cell line was established from the peripheral blood of a multiple myeloma patient who developed resistance to steroid therapy. MM.1R is resistant to dexamethasone. The closely related cell line MM.1S was also isolated from MM.1 but remains sensitive to dexamethasone. After resuscitation, this cell line requires approximately two weeks to resume normal growth.

Cell Name: Human multiple myeloma cell line, dexamethasone-resistant

Cell Synonyms: MM1.R; MM1.r; MM.1R; MM1-R; MM-1R; MM1R; MM1.R(L); MM1.RL

Catalogue No.: TCH-C459
Species: Human
Tissue Origin: Bone Marrow
Disease Characteristics: Peripheral Blood
Morphology: Lymphoblast-like
Growth Characteristics: Semi-adherent/Semi-suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G459
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection