Established from a poorly differentiated invasive esophageal squamous cell carcinoma resected from the cervical canal of a 51-year-old Japanese male prior to treatment (with evident tumor invasion into the adventitia); overexpression of HST-1 (heparin-binding growth factor) has been described in the literature.

Cell Name: Human esophageal squamous cell carcinoma cell line

Cell Synonyms: KYSE410; KYSE 410; KYSE0410

Catalogue No.: TCH-C458
Species: Human
Tissue Origin: Esophagus
Disease Characteristics: Squamous Cell Carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G458
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection