This cell line was derived from squamous cell carcinoma tissue of the tongue of a 25-year-old male. It expresses epidermal keratin and exhibits low levels of integrin. Subcutaneous inoculation of 10⁷ cells into nude mice resulted in tumor formation at a 100% frequency (5/5) within 21 days.

Cell Name: Human tongue squamous cell carcinoma cell line

Cell Synonyms: SCC-9; SCC 9; SCC9

Catalogue No.: TCH-C455
Species: Human
Tissue Origin: Tongue
Disease Characteristics: Squamous cell carcinoma
Morphology: Epithelial-like
Growth Properties: Adherent
Culture System: D/F12 + 10% FBS + 400 ng/mL hydrocortisone + 1% P/S
Matching Medium Catalog Number: TCH-G455

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection