The MDA-MB-231 cell line was established from the pleural effusion of a 51-year-old Caucasian female patient with breast cancer. These cells express epidermal growth factor (EGF) receptors, TGF-α receptors, and the WNT7B oncogene.

Cell Name: Human breast adenocarcinoma cell line

Cell Synonyms: MDA-MB-231; MDA_MB_231; MDA-MB 231; MDA.MB.231; MDA MB 231; MDA MB231; MDA Mb231; MDA-MB231; MDAMB-231; MDAMB231; MDA-231; MDA231; MDA231-BRE; MB231; MD Anderson-Metastatic Breast-231

Catalogue No.: TCH-C453
Species: Human
Tissue Origin: Breast
Disease Characteristics: Breast Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G453

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection