The A-498 cell line was established by Aaronson·S and derived from a 52-year-old female patient with kidney cancer.

Cell Name: Human renal cell carcinoma cell line

Cell Synonyms: A498; A-498

Catalogue No.: TCH-C451
Species: Human
Tissue Origin: Kidney
Disease Characteristics: Kidney carcinoma cells

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G451
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection