The HL-7702 [L-02] cell line is derived from normal human liver cells. The population doubling time of HL-7702 [L-02] cells is approximately 20 hours, and the cells exhibit an epithelial-like morphology. Under electron microscopy, desmosomes and tonofilaments—characteristic features of epithelial cells—are also observed. HL-7702 [L-02] cells proliferate rapidly, show negative expression of AFP and CK-19, and express ALB at a level of 10 μg/ml. Some cells undergo morphological variation after multiple passages, accompanied by loss of ALB expression. (STR profiling matches that of HEK293 or HeLa, indicating potential contamination.)

Cell Name: Human hepatic cell line

Cell Synonyms: L-02; L02; LO2; HL-7702; HL7702

Catalogue No.: TCH-C446
Species: Human
Tissue Origin: Liver

Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G446

Passage Ratio: 1:4-1:8, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection