The HCC1806 breast cancer cell line was initiated on July 31, 1995, and took 10 months to establish.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: HCC1806; Hcc1806; HCC-1806; Hamon Cancer Center 1806

Catalogue No.: TCH-C444
Species: Human
Tissue Origin: Thorax; Breast
Disease Characteristics: Squamous cell carcinoma of the breast

Morphology: Epithelioid, polygonal

Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G444
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection