Cell Name: Immortalized human nasopharyngeal epithelial cell line

Cell Synonyms: NP69; NP69SV40T; NP-69

Catalogue No.: TCH-C440

Species: Human

Tissue Origin: Nasopharynx

Disease Characteristics: —

Morphology: Epithelial-like

Growth Properties: Adherent

Culture Medium: NP69 Specialized Basal Medium + 1% Growth Factors + 1% P/S

Matching Medium Catalog Number: TCH-G440

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection