A cell line derived from undifferentiated nasopharyngeal carcinoma (NPC). This cell line consistently carries Epstein-Barr virus (EBV) during long-term culture. C666-1 is a subclone of its parental cell line C666, originating from NPC xenografts in southern China.

Cell Name: Human nasopharyngeal carcinoma cell line

Cell Synonyms: C666-1; c666-1; C6661

Catalogue No.: TCH-C439

Species: Human

Tissue Origin: Nasopharynx

Disease Characteristics: Nasopharyngeal Carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G439
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection