The lung adenocarcinoma cell line from this patient is available as ATCC CRL-5911. ATCC has confirmed that this cell line is positive for the presence of Epstein-Barr virus DNA sequences as determined by PCR.

Cell Name: EBV-transformed human lymphoblastoid cell lines (LCLs)

Cell Synonyms: NCI-BL2009; BL2009

Catalogue No.: TCH-C433
Species: Human
Tissue Origin: Blood
Disease Characteristics: —
Morphology: Lymphoblast
Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G433

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection