The NCI-H520 cell line was established in 1982 by Gazdar AF et al., and is derived from squamous cell lung carcinoma tissue. The expression level of p53 mRNA in NCI-H520 cells is lower than that in normal lung tissue, but there are no major structural abnormalities in DNA. NCI-H520 cells are positive for keratin and vimentin, but negative for neurofilament triplet proteins. NCI-H520 cells can form colonies in soft agar.

Cell Name: Human lung squamous cell carcinoma cell line

Cell Synonyms:NCI-H520; H520; H-520; NCI-HUT-520; NCIH520

Catalogue No.: TCH-C427

Species: Human
Tissue Origin: Lung
Disease Characteristics: Lung squamous cell carcinoma

Morphology: Lymphoblast-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS +1% P/S
Matching Medium Catalog Number: TCH-G427
Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection