The NCI-H524 cell line was established in 1982 and derived from metastatic lymph nodes of a male patient with non-small cell lung cancer.

Cell Name: Human small cell lung carcinoma cell line

Cell Synonyms: NCI-H524; H524; H-524; NCIH524

Catalogue No.: TCH-C423
Species: Human

Tissue Origin: Lung
Disease Characteristics: —
Morphology: Lymphocyte-like
Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G423

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection