The JEG-3 cell line is a hypertriploid human cell line. The typical chromosome number of JEG-3 cells is 71, with an incidence rate of 34%, and the polyploidy rate is 2.6%. Structural chromosomal abnormalities commonly found in most cells include t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21), and six other marker chromosomes. Two additional markers are observed in some cells, and large satellites are present on one N14 and two N22 chromosomes. Chromosomes N2, N5, and N9 have four copies each, while N7, N13, N18, N21, and the X chromosome are single-copy. A single Y chromosome can be detected using Q-band staining.

Cell Name: Human choriocarcinoma cell line

Cell Synonyms: JEG-3; Jeg-3; JEG3; Jeg3; jeg3

Catalogue No.: TCH-C417
Species: Human
Tissue Source: Placenta
Disease Characteristic: Choriocarcinoma

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G417

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection