Cell Name: Human non-small cell lung adenocarcinoma cell line

Cell Synonyms: NCI-H2009; H2009; H-2009; NCIH2009

Catalogue No.: TCH-C415

Species: Human
Tissue Origin: Lung
Disease Characteristics: Lung adenocarcinoma

Morphology: Lymphoblast-like
Growth Properties: Adherent

Culture Medium: D/F12+10% FBS+1% Glutamax + 10 nM beta-estradiol + 10 nM Hydrocortisone + 30 nM Sodium selenite + 0.01 mg/mL Transferrin + 0.005 mg/mL Insulin + 1% P/S
Matching Medium Catalog Number: TCH-G415
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection