The NB-4 cell line was derived from the bone marrow of a patient with acute promyelocytic leukemia (APL = AML FAB M3) during the second relapse in 1989.

Cell Name: Human acute promyelocytic leukemia cell line

Cell Synonyms: NB4; NB-4; NB.4

Catalogue No.: TCH-C413
Species: Human
Tissue Origin: Bone Marrow
Disease Characteristics: Leukemia
Morphology: Lymphoblast-like
Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G413

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection