$ 1000.00
Human mammary epithelial cell line(MCF-12A)
Cat.No. Size
  • Parameters
  • Detail
  • Cell Info
  • References
Cat.No.
TCH-C411
Size
1x10^6
Price
$1000.00

MCF-12A is an epithelial cell line isolated from the breast tissue of a 60-year-old Caucasian female patient. The MCF-12A cell line is a non-tumorigenic epithelial cell line derived from tissue obtained during a reduction mammoplasty from a patient with fibrocystic breast disease featuring focal areas of ductal hyperplasia.



Cell Name: Human mammary epithelial cell line

Cell Synonyms: MCF-12A; MCF12A; Michigan Cancer Foundation-12A

Catalogue No.: TCH-C411
Species: Human
Tissue Origin: Breast
Disease Characteristics: Breast Cancer
Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: D/F12 + 20 ng/mL Human EGF + 100 ng/mL Cholera Toxin + 500 ng/mL Hydrocortisone + 5% Horse Serum + 1% P/S
Matching Medium Catalog Number: TCH-G411
Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection


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