This cell line was isolated in 1993 from the gastric lymph nodes of a 35-year-old female patient with signet ring cell carcinoma. The value of NUGC-4 cells in basic and preclinical research lies in their broad range of applications, particularly in in-depth studies on the pathological characteristics of signet ring cell carcinoma and the development of therapeutic intervention strategies.

Cell Name: Human gastric carcinoma cell line

Cell Synonyms: NUGC-4; NUGC4; NU-GC-4; Nagoya University-Gastric Cancer-4

Catalogue No.: TCH-C408
Species: Human
Tissue Origin: Stomach
Disease Characteristics: Gastric Cancer
Morphology: Mixed growth pattern, including both lymphoblast-like suspension and adherent epithelial-like cells
Growth Properties: Semi-adherent / semi-suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G408
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma detection