Product Name：Human Umbilical Vein Endothelial Cell Line

HyCyte cell line：HUVEC

Cat.No.：TCH-C406

Organism：Homo sapiens, human

Organizational Sources：Umbilical cord tissue

Morphology：Endothelial

Growth Properties：Adherent

Culture System：Endothelial cell basic medium + 5% FBS (fetal bovine serum) + endothelial cell culture supplement + 1% P/S (dual antibiotics). It is recommended to use the HyCyte® complete culture medium for HUVECs , Cat. No. TCH-G406.

Digestion Time：

0.25% trypsin (0.04% EDTA) digests at 37℃ for 1-2 minutes. Note: The digestion time may vary slightly with different brands of trypsin and cell densities, and should be based on the majority of cells rounding up and detaching.

Note：

1. The presence of a small amount of black particles and dead cell debris during cell culture is a normal phenomenon and can be removed by changing the medium.

2. Using HyCyte® complete culture medium, passaging at a ratio of 1:2, it is promised that it can be passaged for an additional five generations after receipt.

3. This cell line is a finite cell line; it is recommended to conduct experiments as soon as possible after receipt, and cryopreservation for preservation is not advised.

Passage Ratio：1:2（based on flask bottom area）, Change the fluid every 2-3 days

Culture Environment：Gas phase: 95% air + 5% carbon dioxide, temperature: 37°C

Cryopreservation Conditions：Cryopreservation conditions: 60% basal medium + 30% FBS + 10% DMSO Recommended HyCyte^® One Step Freezing Medium, Cat. No. GUCP-R201.

Storage conditions: Liquid nitrogen storage

Safety：

All tumour cells and virus-transfected cells are considered potentially biohazardous, and it is recommended to operate in a biosafety level 2 station with personal protection.