Human embryonic kidney cells 293F [HEK293-F], also known as HEK 293-F, HEK 293F, 293-F, etc. Human embryonic kidney cells 293F [HEK293-F] (where F stands for Fast-growth) are a rapidly growing, highly transfectable clonal strain derived from 293 [HEK-293] cells (Catalog No.: SCSP-5209). These cells retain the epithelial-like morphology of the parental 293 cells, adapt to various growth conditions, and exhibit suspension growth in specific serum-free media without attachment to solid substrates. The 293F cells are highly efficient in expressing recombinant proteins and are widely used for preparing viral vectors for gene therapy, including adenovirus, lentivirus, and retrovirus vectors. They grow rapidly, are easily transfected, and are particularly suitable for transient transfection, enabling high-yield protein production within days post-transfection. This characteristic facilitates scale-up processes in biotechnology and biopharmaceuticals.

Cell Name: Human Embryonic Kidney Cells (STR-verified)
Cell Synonyms: HEK-293-F; HEK 293-F; HEK-293F; HEK293F; 293-F; 293 F; 293F

Catalogue No.: HEK293-F

Species: Human
Tissue Origin: Embryonic kidney
Disease Characteristics: /
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS (Fetal Bovine Serum) + 1% P/S
Matching Medium Catalog Number: TCH-G405
Passage Ratio: 1:2–1:4, change medium every 2–3 days
Doubling Time: 24–36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma