The Bewo cell line was derived from human choriocarcinoma tissue that had metastasized to the brain. It was serially passaged for 8 years through transplantation into the cheek pouches of hamsters. The resulting transplant tumor tissue was cultured in vitro to establish the cell line. Different sublines were developed using various passage methods, with JEG-3 being one of its derivative clones. Bewo cells are capable of producing hormones such as estrogen, progesterone, estrone, estradiol, estriol, hCG (human chorionic gonadotropin), placental lactogen, as well as keratin.
Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days Doubling Time: 56-84 h Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201 Quality Control: Negative for bacteria, fungi, and mycoplasma detection
Precautions:
These cells exhibit a sheet-like growth pattern with relatively poor migratory ability. Their growth characteristic involves gradual expansion from the center outward. During subculturing, maintain a split ratio between 1:2 and 1:3.
Excessive split ratios may cause over-confluence and detachment of the central portion of cell sheets. If significant cell floating is observed during culture, check whether the split ratio is too high and adjust accordingly.
When overall confluence is low but central over-confluence of cell sheets causes excessive floating cells, replating may be performed. Adjust to moderate confluence to facilitate optimal cell growth.