Derived from normal follicular cells of the ovarian tissue bank at the University of British Columbia, established as a cell line through transfection with SV40 immortalizing virus.

Cell Name: Immortalized human ovarian surface epithelial cell line

Cell Synonyms: IOSE-80; IOSE 80; IOSE80; NFHIOSE-80; IOSE80UBC; IOSE-Van; IOSE-VAN

Catalogue No.: TCH-C402

Species: Human

Tissue Origin: Ovary

Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G402

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection