Thymidine kinase negative (TK-). This is a human osteosarcoma cell line.

Cell Name: Human osteosarcoma cell line, TK-deficient

Cell Synonyms: 143B; 143b; 143 B; 143B TK-; 143B.TK-; 143BTK-; 143TK-; HOS-143B; HOS-143b; GM05887; GM05887A

Catalogue No.: TCH-C401
Species: Human
Tissue Origin: Bone
Disease Characteristics: Osteosarcoma

Morphology: Fibroblast-like and epithelial-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 1% NEAA + 1% P/S
Matching Medium Catalog Number: TCH-G401

Passage Ratio: 1:2, with medium renewal every 2-3 days

Doubling Time: 36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection