The HMC3 cell line was established through SV40-dependent immortalization of a human fetal brain-derived primary microglial culture.

Cell Name: Human microglial cell line (STR-authenticated)
Cell Synonyms: HMC3
Catalogue No.: TCH-C399
Species: Human
Tissue Origin: Brain
Disease Characteristics: —
Morphology: Macrophage-like

Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G399

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection