This cell line was derived from a 24-year-old Asian male. The cells contain a single nucleus, and hepatitis B virus (HBV) DNA can be detected by Southern blot; however, hepatitis B viral genomic RNA is not expressed. Handling requires Biosafety Level 2 (BSL-2) precautions.

Cell Name: Human hepatocellular carcinoma cell line (STR profile confirmed)
Cell Synonyms: SNU-182; SNU182; NCI-SNU-182

Catalogue No.: TCH-C398
Species: Human

Tissue Origin: Liver
Disease Characteristic: Hepatocellular carcinoma

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% Sodium Pyruvate＋1% Glutamax+1% P/S
Matching Medium Catalog Number: TCH-G398

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection