The MKN-45 cell line was established by S. Akiyama and derived from the gastric lymph nodes of a 62-year-old female patient with signet ring cell carcinoma.

Cell Name: Human gastric carcinoma cell line

Cell Synonyms: MKN-45; MKN 45

Catalogue No.: TCH-C397
Species: Human
Tissue Origin: Stomach
Disease Characteristic: Gastric Cancer
Morphology: Lymphocyte-like
Growth Properties: Semi-adherent / Semi-suspension

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G293

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection