This cell line was derived from the normal skin at the periphery of a lesion in a 62-year-old male patient with melanoma. It is positive for keratin, involucrin (involucrin is an epidermal cornified envelope protein), and filaggrin (filament-associated protein).

Cell Name: Immortalized human keratinocyte cell line

Cell Synonyms: HaCaT ; HaCAT; HACAT; Hacat

Catalogue No.: TCH-C388

Species: Human

Tissue Origin: Skin

Disease Characteristics: --

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G388

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection