The LN-229 cell line was established in 1979 from cells taken from a patient with a right frontoparietal occipital glioblastoma.

Cell Name: Human glioblastoma cell line

Cell Synonyms: LN-229; LN 229; LN229; LNT-229

Catalogue No.: TCH-C387
Species: Human
Tissue Origin: Brain
Disease Characteristics: Glioblastoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 5% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G387

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection