Disease Characteristics: --

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 3.3 nM EGF + 400 nM hydrocortisone + 870 nM insulin + 1% P/S
Matching Medium Catalog Number: TCH-G386

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection