Product Name：Luciferase/GFP Dual-Reporter PC-9 Cell Line

HyCyte cell line：HyCyte PC-9-Luc-GFP

Catalog No.：TCH-C380LG

Construction method：PiggyBac

Selection Marker：Hygro B

Parental：PC-9

Species：Human

Morphology：Rounded & Epithelioid

Growth properties：Adherent

Culture Medium：RPMI-1640 supplemented with 10 % FBS,2.0ug/mL Puromycin,   2mM Glutamax, 1mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G380L).

Passage：1:4-1:6

Doubling time：24-32 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).