This cell line was derived from a 37-year-old Caucasian male patient with glioma.

Cell Name: Human neuroglioma cell line

Cell Synonyms: H4; H-4

Catalogue No.: TCH-C379

Species: Human

Tissue Origin: Brain

Disease Characteristics: Glioma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G379

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection