The ZR-75-1 cells produce high levels of mucin MUC-1 mRNA, low levels of MUC-2 mRNA, but do not express the MUC-3 gene; the ZR-75-1 cells express estrogen receptors.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: ZR-75-1;ZR751; ZR75-1; ZR75_1

Catalogue No.: TCH-C375
Species: Human
Tissue Origin: Breast
Disease Characteristics: Breast Cancer
Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G375
Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection