The WERI-Rb-1 cell line is one of two human eye cancer cell lines established in 1974 by R.M. McFall and T.W. Sery. The cells can survive in Difco Bacto-Agar but do not form colonies.

Cell Name: Human retinoblastoma cell line

Cell Synonyms: WERI-RB-1; WERI-Rb-1; WERI-Rb 1; WERI-Rb1; WERI-RB1; WERI Rb-1; WERIRb1; WERI; Wills Eye Research Institute-Retinoblastoma-1

Catalogue No.: TCH-C371

Species: Human

Tissue Origin: Retina

Disease Characteristic: Retinoblastoma

Morphology: Round

Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G371

Passage Ratio: 3×10⁵-5×10⁵ cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection